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rabbit polyclonal anti pen2  (Danaher Inc)


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    Danaher Inc rabbit polyclonal anti pen2
    Rabbit Polyclonal Anti Pen2, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+anti+pen2/10__7554_slash_elife__56679-320-37-41
    Average 86 stars, based on 1 article reviews
    rabbit polyclonal anti pen2 - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Western Blot:

    Article Title: Super-resolution microscopy reveals majorly mono- and dimeric presenilin1/γ-secretase at the cell surface
    Article Snippet: For confocal microscopy, we used the rat monoclonal anti-LAMP1 (sc-19992, Santa Cruz, 1:200). .. For WB, the following antibodies were used: mouse monoclonal anti-NCT (Esselens et al., 2004) (9C3, 1:7000), rabbit polyclonal anti-PSEN1-NTF (ab71181, Abcam, 1:2000) or rat polyclonal anti-PSEN1NTF for co-immunoprecipitation (MAB1563, Millipore, 1:4000), rabbit monoclonal anti-PSEN1-CTF (ab76083, Abcam, 1:2000), rabbit polyclonal anti-PEN2 (ab18189, Abcam, 1:1000), rabbit polyclonal anti-APP-CTF (Esselens et al., 2004) (B63, 1:10,000) and mouse monoclonal anti-transferrin receptor (136800, Invitrogen, 1:4000). .. Secondary antibodies included HRP-conjugated goat-anti-mouse and goat-anti-rabbit (Bio-Rad, 1:10000 dilution).



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    Image Search Results


    RAP1 and GFAPɛ colocalize with subunits of the γ-secretase complex. SH-SY5Y cells were grown, fixed, and probed with antibodies as indicated in each panel. Representative images are shown that include images of each fluorophore individually with a larger, merged image. The box in the merged images indicates the region that is increased in size for the inset at the top right corner. Arrows in the inset indicate examples of colocalization. a) RAP1 and GFAPɛ colocalize. Cells were probed with DAPI, antibodies to RAP1 and antibodies to GFAPɛ. b) RAP1 colocalizes with the γ-secretase subunit PSENEN. Cells were probed with DAPI, antibodies to RAP1, and antibodies to PSENEN. c) RAP1 and GFAPɛ colocalize with the γ-secretase subunit PS1. DAPI stain was not used in this image. Cells were probed with antibodies to PS1, antibodies to RAP1 and antibodies to GFAPɛ.

    Journal: G3: Genes|Genomes|Genetics

    Article Title: The human RAP1 and GFAPɛ proteins increase γ-secretase activity in a yeast model system

    doi: 10.1093/g3journal/jkad057

    Figure Lengend Snippet: RAP1 and GFAPɛ colocalize with subunits of the γ-secretase complex. SH-SY5Y cells were grown, fixed, and probed with antibodies as indicated in each panel. Representative images are shown that include images of each fluorophore individually with a larger, merged image. The box in the merged images indicates the region that is increased in size for the inset at the top right corner. Arrows in the inset indicate examples of colocalization. a) RAP1 and GFAPɛ colocalize. Cells were probed with DAPI, antibodies to RAP1 and antibodies to GFAPɛ. b) RAP1 colocalizes with the γ-secretase subunit PSENEN. Cells were probed with DAPI, antibodies to RAP1, and antibodies to PSENEN. c) RAP1 and GFAPɛ colocalize with the γ-secretase subunit PS1. DAPI stain was not used in this image. Cells were probed with antibodies to PS1, antibodies to RAP1 and antibodies to GFAPɛ.

    Article Snippet: The samples were first incubated with primary antibodies to GFAPɛ (described above) or PSENEN (rabbit polyclonal anti-PEN2 antibodies, Invitrogen, PA5-20302) in blocking solution.

    Techniques: Staining